Synechocystis was Engineered to have a Green-Light-Inducible Gene Expression Mechanism
Department of Restorative Dental Sciences, College of Dentistry, Imam Abdurrahman Bin Faisal University, Dammam
31441, Saudi Arabia
itation: Gou Dong, Jian Xiao, Abdul Samad Khan, “Synechocystis was Engineered to have a Green-Light-Inducible
Gene Expression Mechanism”, American Research Journal of Biotechnology, Vol 1, no. 1, 2022, pp. 38-41.
Abstract
To develop a green light-regulated gene expression system unique to cyanobacteria, the CcaS histidine kinase, CcaR
response regulator, and cpcG2 gene promoter of Synechocystis PCC6803 must be discovered (PcpcG2). Using a genetic
controller to shine green light on CcaS and CcaR might activate the PcpcG2 gene. Researchers studied the capacity of the
native PcpcG2 to react to green light using the reporter gene GFPuv. Because of this, it was evident that green light had
a direct effect on both the amount of expression of PcpcG2 and the amount of expression of Ptrc, another cyanobacteria
specific constitutive promoter. The insertion of a ShineDalgarno-like motif into cpcG2’s 5′ untranslated region increased
CcaR expression. With this improved green-light detector in place, protein expression increased by a factor of 40 under
green light irradiation. Bioprocesses that utilise novel cyanobacteria may benefit from the utilization of green-light gene
expression systems
