The Chromosomal DNA Position of Amplified Genes Affects Recombinant Protein Production and Gene Stability
1Department of Electrical and Electronics Engineering, KocUniversity ,Sar?yer, Istanbul, 34450, Turkey.
2Graduate School of Science and Technology, Koc University, Sar?yer, Istanbul, 34450, Turkey.
3Surface Science and Technology Center, Koc University, Sar?yer, Istanbul, 34450, Turkey.
Citation: Onur Ergen, Ecem Celik, Ahmet Hamdi Unal, “The Chromosomal DNA Position of Amplified Genes Affects
Recombinant Protein Production and Gene Stability”, American Research Journal of Biotechnology, Vol 1, no. 1, 2022,
pp. 42-49.
Abstract
In the past, in order to produce a genetically expanded CHO cell line that was not only extremely durable but also
extremely prolific, we devised a procedure that could be carried out in a short amount of time, required little in the way
of supervision, and was straightforward to carry out. This allowed us to generate a genetically expanded CHO cell line
that was both exceptionally long-lasting and extremely prolific. Methotrexate (MTX) at higher concentrations led to the
production of gene-amplified cell populations, which exhibited high and constant rates of specialized growth and output.
These gene-amplified cell populations were shown to be resistant to the methotrexate. It would seem that the part of the
chromosome’s DNA that contained the bigger gene was responsible for the phenotypic characteristics that were shown
by cells that had their genes amplified. We predicted that as we progressed through our exhaustive process of selection,
a variety of unique gene-amplified cell types would eventually stand out to us as the most promising candidates. These
clones were obtained from gene-amplified cell pools. This was done in order to get a deeper comprehension of how gene
amplified cell pools respond to a gradual rise in the amount of MTX present in the environmentThis led to the discovery of
“telomere-type clones,” which, in comparison to their conventional analogues, were more stable and produced a greater
quantity of offspring. Following an analysis of the chromosomal DNA of telomere-type clones, it was found that these
clones included more than one hundred copies of amplified genes. It is not unheard of to find less than 10 copies of
amplified genes amongst a significant number of other clones; this is not an extremely rare occurrence. This is by no
means something that happens just seldom. When, on the other hand, amplified genes were cultured for an extended
period of time in other types of clones, MTX was not present; as a direct result, there was a rapid loss of the genes from
the chromosomal DNA.
