Gelatin Coated Albumin Nano Particles Bearing Acyclovir for Effective Management of Visceral Leishminiasis by Targeting Liver Kupffer Cells
1*Shri Guru Ram Rai Institute of Technology and Sciences, Patel Nagar, Dehradun
1Shri Guru Ram Rai Institute of Technology and Sciences, Patel Nagar, Dehradun
Abstract
To make drug comfortable for administration to patients it is converted to dosage form by mixing with
recipients, additives. Nano particles are one of the novel colloidal drug delivery systems that hold great promise
for reaching the goal of controlled drug delivery as well as site-specific delivery. In the present study, an attempt
was made to develop gelatin coated albumin nano particles of acyclovir for the treatment of visceral leishmaniasis
with a view to see the targeting of nano particles at the required site for action and helps to provide sustain release
and thus reduces the dose frequency and increase the patient compliance. Pre formulation testing is the first step
in the rationale development of dosage form o a drug .The method was able to produce the free flowing nano
particles. Bovine serum albumin is a biodegradable polymer for preparing targeted nano particle. FTIR studies
were carried out, revealed that there was no interaction between the selected drug and polymer. The particle size
analysis revealed that the particle size was found to be in the size range of 200-800 nm ,that comes under nano
range. The zeta potential of nano particle was found to be -59.3, having good stability. The maximum entrapment
efficiency was found to be 86.78±0.5 % for the formulation F3. The entrapment efficiency increases with
increasing polymer concentration but upto a certain ratio. The maximum percentage yield was found to be
51.13±0.53% for formulation F4, where the concentration of albumin is highest while nano particles yield is
lowest in F1 19.10±0.79.as the concentration of albumin is lowest. In vitro release shown as, cumulative release
of drug was determined up to 8 hrs for formulationF1, F2,F3,F4 was 62.48± 0.38,51.67± 0.55,48.34± 0.29,34.98±
0.32 respectively. The receptor ligand binding is 95.89 %. And it was confirmed by checking the amount of drug
release from formulation is 3.89% while prior it was 48.34%, so the remaining drug was bind with receptor cells
